作者
Carlos Alfonso-Gonzalez, Laura Arrigoni, Hasan Can Ozbulut, Stefanie Falk, Ulrike Bönisch, Valérie Hilgers
发表日期
2023/12/15
期刊
STAR protocols
卷号
4
期号
4
页码范围
102505
出版商
Elsevier
简介
We present a detailed protocol for sequencing full-length mRNA isoforms using the Oxford nanopore long-read sequencing technology. We describe steps for poly(A) RNA isolation, library preparation, and cDNA size selection. We then detail procedures for sequencing and processing and a computational framework to identify exon couplings and assign mRNA 5′ ends and 3′ ends to each other. Our approach enables the identification of links between transcription initiation and co-transcriptional RNA processing events.
For complete details on the use and execution of this protocol, please refer to Alfonso-Gonzalez et al.1
学术搜索中的文章
C Alfonso-Gonzalez, L Arrigoni, HC Ozbulut, S Falk… - STAR protocols, 2023