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Microalgae-derived bioactive bioproducts are due to their potential application in pharmaceuticals and nutraceuticals. Nevertheless, the exorbitant cost of nutrients essential for microalgal cultivation is a significant obstacle to commercially feasible algal-mediated value-added compound manufacturing. Fruit waste like banana peel and sweet lime peels were used instead of traditional growing media in this investigation to cultivate Monoraphidium sp. These wastes are zero-value substances comprising essential nutrients and components to produce microalgae biomass. Response surface methodology (RSM) based on a central composite design (CCD) was used to analyze the optimization of fruit waste slurry with nitrogen content on biomass, lipids, and carotenoids production. When the Monoraphidium sp. was grown in 12.5 ml L⁻¹ banana peel slurry (BPS) and 2.5 ml L⁻¹sweet lime peel slurry (SLPS) with 1.5 g …