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The source of biodiesel production is significant as the demand for diesel is very high. Sustainable fuel development is the prime aim of meeting the demand. Drought-tolerant trees are widely available and can cultivate more to increase the feedstock capacity. Hence, this experimental research investigates the potential for deriving an alternative fuel from borassus flabellifer. Accordingly, this research employed biodiesel production from borassus flabellifer oil through the transesterification technique with methanol at 65 °C of temperature for 3 h with 300 rpm of magnetic stirrer speed. The blends by volume of 20% biodiesel of borassus flabellifer (20BOPP) and 80% of diesel (80D) were used to create the Biodiesel of borassus flabellifer blend (20BOPP+80D). Then it is enhanced by mixing 100 ppm of aluminium oxide nanoparticles (AONP) in that fuel to produce the nano-fuel of 20BOPP+80D + AONP. The base fuel …