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The generation of hematopoietic stem cells (HSCs) from human pluripotent stem cells (hPSCs) is a major goal for regenerative medicine, as it will provide an unlimited source of these cells for transplantation, and, a unique platform for the study of both normal and disease hematopoietic processes. To reproducibly achieve this goal in all hPSC lines, we must first fully understand hematopoietic ontogeny. We have recently developed a novel method to obtain pure KDR+ CD235a+ primitive or pure KDR+ CD235a-definitive progenitors from hPSCs through stage-specific manipulation of Wnt signaling. We performed whole-transcriptome gene expression analyses on these isolated populations and identified strong CDX and HOX gene enrichment within KDR+ CD235a-definitive hematopoietic mesoderm. CDX1 and CDX2 were expressed in broad mesendoderm, while CDX4 was expressed later exclusively within …