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Mitogen activated protein kinase (MAP kinase) signal transduction pathways are important eukaryotic mechanisms for regulating cellular responses to stress. The objective of this work was to investigate the role of the barley MAP kinase HvMPK4 (a homologue of the Arabidopsis MAP kinase AtMPK1) in the plant response to biotic and abiotic stress. Transgenic barley plants bearing antisense or overexpression constructs for HvMPK4 were produced, and RNA blot analysis showed that HvMPK4 gene expression was much reduced in the antisense lines and approximately double in the overexpression lines. Three independent lines of each construct were tested for their response to a fungal pathogen and to salt treatment. The antisense lines were more resistant to the hemibiotrophic fungal pathogen Magnaporthe grisea, and showed enhanced levels of salicylic acid (SA) and of hydrogen peroxide following infection …