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Materials and methods

The present study includes 290 acute leukaemia cases comprising of 147 acute lymphocytic leukaemia (ALL), 143 acute myeloid leukaemia (AML), 248 healthy age and sex matched individuals without family history of leukaemia or any other cancers were selected to serve as control group. Five mL of blood sample were collected into EDTA vacutainers from acute leukaemia patients being reported at NIMS (Nizam’s Institute of Medical Sciences), Hyderabad, either before starting chemotherapy or after remission. The age and sex matched control samples were randomly selected from different areas of the local population. Patient’s clinical data like WBC count, blast%, platelet count, Hb, LDH, complete remission (CR) response to therapy and disease free survival (DFS) were noted from the tumour registry file with the help of medical oncologist. Genomic DNA was isolated using salting-out method (Nuremberg and Lahari 1991)