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CelE from Clostridium thermocellum is a glycoside hydrolase family 5 enzyme that has broad specificity for hydrolysis of plant biomass polysaccharides, particularly cellulose, xylan, and mannan. A fusion of CelE with the well-studied carbohydrate binding module CBM3a was created, CelE_CBM3a, and this construct alone was able to give high-yield hydrolysis of both cellulose and hemicellulose in pretreated plant biomass. In this study, thirty-nine different CBMs from the C. thermocellum genome were fused to GFP, and the specific binding interactions of the robotic cell-free translated GFP_CBM constructs were determined with a panel of soluble and insoluble substrates. Most CBMs tested were able to bind at least one substrate, and several GFP_CBMs displayed unanticipated binding capabilities. We also asked whether these CBMs could yield different catalytic capabilities when fused to the multifunctional …