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Carbon dioxide (CO₂) acts as a weak acid in water and the increasing level of CO₂ in the atmosphere leads to ocean acidification. In addition, possible leakage from sub-seabed storage of anthropogenic CO₂ may pose a threat to the marine environment. ¹H NMR spectroscopy was applied to extracts of hemolymph, gills and leg muscle from shore crabs (Carcinus maenas) to examine the metabolic response to elevated levels of CO₂. Crabs were exposed to different levels of CO₂‐acidified seawater with pH_NBS 7.4, 6.6 and 6.3 (pCO₂~2600, 16,000 and 30,000μatm, respectively) for two weeks (level-dependent exposure). In addition, the metabolic response was followed for up to 4weeks of exposure to seawater pH_NBS 6.9 (pCO₂~7600μatm). Partial least squares regression analysis of data showed an increased differentiation between metabolic fingerprints of controls and exposed groups for all sample types …