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In this study, we determined the protective potential of trans resveratrol against oxygen–glucose deprivation (OGD) induced reactive oxygen species mediated apoptotic damages in PC12 cells. In vitro model of ischemic cerebral stroke was created by keeping cells in an OGD condition for 6 h followed by 24 h reoxygenation. Cells received biologically safe doses (5, 10, and 25 μM) of trans resveratrol in the following schedules for 24 h prior to OGD; during 6 h of OGD; for 24 h post OGD and whole treatment group which starts from 24 h before OGD and lasted to 24 h post OGD. Anti-ischemic potential of trans resveratrol was assessed by measuring the regulation of lipid peroxidation, reactive oxygen species production, glutathione content, and expression (mRNA and protein) of apoptotic markers such as Bax, Bcl₂ and Caspase-3. Hypoxia inducible factor-1α (HIF-1α) was also assessed to correlate the changes with …