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Poultry is the second largest industry of Pakistan but its progress was hampered due to many bacterial and viral diseases which are major constraints for profitable poultry production (Ahmed et al., 2009). Among viral diseases, the most significant is Newcastle disease (ND). Newcastle disease virus (NDV) is designated APMV-1. NDV is grouped into five pathotypes (Alexander and Senne, 2008; Alexander, 2010), which include Viscerotropic, Neurotropic, Velogenic, Mesogenic and Lentogenic. Since its acknowledgment in 1926, ND is counted as being endemic in many countries. Regardless of vaccination, other prevention and control measures are necessary to prevent ND outbreaks. Every year, ND causes destruction of poultry industry in Pakistan, hence affects the economy of country badly. Due to aerosol transmission and the resistance of the virus in the environment, NDV causes infection in other birds (Rasool et al., 2015; Pansota et al., 2013; Sattar et al., 2016).