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Pre-storage treatment of superficial scald-susceptible apple and pear fruits with the blocker of ethylene action 1-methylcyclopropene (1-MCP) inhibits the synthesis of α-farnesene. Consequently, accumulation of α-farnesene and its conjugated trienol (CTol) oxidation products in the fruit skin is diminished, and scald is largely prevented. In cold-stored apple fruit, a marked increase in expression of AFS1, the gene encoding α-farnesene synthase (AFS), precedes the rapid accumulation of α-farnesene. A Pyrus communis L. gene encoding AFS (PcAFS1) was cloned using RT-PCR with primers based on apple AFS1 and RNA from peel tissue of ‘d’Anjou’ pears cold-stored for 33–94 days. Non-treated control and 1-MCP-treated (300nLL⁻¹ for 6h at 1°C) pears were stored at −1°C in air for up to 216 days. PcAFS1 expression in control fruit increased sharply over the first 63 days, remained high through 123 days, fell to …