作者
Judith Magenheim, Ariel Rokach, Ayelet Peretz, Netanel Loyfer, Gordon Cann, Hamed Amini, Patriss Moradi, Sudharani Nagaraju, Wafa Sameer, Assaf Cohen, Ophir Fogel, Rottem Kuint, Avraham Abutbul, Aiman Abu Rmeileh, Mutaz Karameh, Polina Cohen Goichman, Ori Wald, Amit Korach, Daniel Neiman, Ilana Fox-Fisher, Joshua Moss, Daniel Cohen, Sheina Piyanzin, Roni Ben Ami, Ahmad Quteineh, Eliahu Golomb, Ruth Shemer, Benjamin Glaser, Tommy Kaplan, Zvi G Fridlender, Yuval Dor
发表日期
2022/11/1
期刊
European Respiratory Journal
卷号
60
期号
5
出版商
European Respiratory Society
简介
Background
Circulating biomarkers for lung damage are lacking. Lung epithelium-specific DNA methylation patterns can potentially report the presence of lung-derived cell-free DNA (cfDNA) in blood, as an indication of lung cell death.
Methods
We sorted human lung alveolar and bronchial epithelial cells from surgical specimens, and obtained their methylomes using whole-genome bisulfite sequencing. We developed a PCR sequencing assay determining the methylation status of 17 loci with lung-specific methylation patterns, and used it to assess lung-derived cfDNA in the plasma of healthy volunteers and patients with lung disease.
Results
Loci that are uniquely unmethylated in alveolar or bronchial epithelial cells are enriched for enhancers controlling lung-specific genes. Methylation markers extracted from these methylomes revealed that normal lung cell turnover probably releases cfDNA into the air spaces …
学术搜索中的文章
J Magenheim, A Rokach, A Peretz, N Loyfer, G Cann… - European Respiratory Journal, 2022