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In this study, the levels of organochlorine (gamma-BCH, Alpha-BCH, aldrin, omicron, rho'-DDE, endosulfan, dieldrin, p, p'-DDT, lindane, p, p'-DDD, omicron, rho'-DDD and methoxychlor) pesticide residues in Alau Dam from Konduga Local Government, Borno State, Nigeria, was investigated using fish samples as a case study. Four fish species (Ciarias gariepinus, Heterotis niloticus, Oreochromis niloticus and Tilapia zilli) were collected for this analysis. Fish samples of uniform size were collected in order to avoid possible error due to size differences. The fish samples were labeled with a unique identification number. Samples of fishes were transported to the laboratory on the same day and later dissected to remove the flesh, liver, stomach and Gills of each species offish and store using 4% formalin pending extraction and analysis. The extraction, clean up and de-fattening of the fishes organs were carried out using …