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Methicillin-Resistant Staphylococcus aureus (MRSA) is one of the most typical bacteria Infectious in Libyan hospitals. Therefore, the present study was conducted as an attempt to find an alternative treatment to reduce the infection MRSA, from crude acetone and ethanol extracts of brown algae (Padina pavonica and Sargassum vulgare) against three MRSA isolates: Wound (W), Skin (S) and diabetic foot ulcers (DFU), by comparing with three an antibiotics (Imipenem, Gentamicin and Tetracycline), the drug susceptibility test was performed using a disc diffusion technique. The obtained results showed that all extracts of brown algae showed a clear inhibition effect toward the growth of two isolates of MRSA (W) and (S). On the other hand, P. pavonica extracts are more efficient than S. vulgare extracts, especially acetone, MARSA (DFU) was the most resistant isolates to antibiotics and extracts. Imipenem showed good activity against a tested MRSA species, which is a noteworthy results. Generally, it can be concluded that acetone extracts of P. panovica may be a good source of compounds effective for anti-MRSA.