查看文章

Development of neuroprotective strategies for peripheral neuropathies requires high‐throughput drug screening assays with appropriate cell types. Currently, immortalized dorsal root ganglion (DRG) sensory neuronal cell lines that maintain nociceptive sensory neuronal properties are not available. We generated immortalized DRG neuronal lines from embryonic day 14.5 rats. Here, we show that one of the immortalized DRG neuronal lines, 50B11, has the properties of a nociceptive neuron. When differentiated in the presence of forskolin, these cells extend long neurites, express neuronal markers, and generate action potentials. They express receptors and markers of small‐diameter sensory neurons and upregulate appropriate receptor populations when grown in the presence of glial cell line–derived neurotrophic factor or nerve growth factor. Furthermore, they express capsaicin receptor transient receptor …