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Objective: the objective of the study is to measure safety culture in an Iranian hospital. Method: The present descriptive study was carried out through a cross-sectional method during spring of 2008. Standard AHRQ questionnaire on patient safety culture survey was applied. Study population comprised of the hospital nursing staff. The survey was carried out on 239 nursing staff. Results: Supervisor expectations and actions promoting patient safety and teamwork within units were highest scored dimensions of hospital regarding patient safety. Among hospital weaknesses regarding patient safety, no punitive response to error and overall patient safety grade were most brilliant. Conclusion: The hospital can establish a safe environment by trying to overcome its weaknesses. Creating an events reporting system and encouraging personnel to report probable errors and events and taking non punitive actions is suggested to treat events.