作者
Thomas Koelblen, Célia Bergé, Mickaël V Cherrier, Karl Brillet, Luisa Jimenez‐Soto, Lionel Ballut, Junichi Takagi, Roland Montserret, Patricia Rousselle, Wolfgang Fischer, Rainer Haas, Rémi Fronzes, Laurent Terradot
发表日期
2017/12
期刊
The FEBS Journal
卷号
284
期号
23
页码范围
4143-4157
简介
The more severe strains of the bacterial human pathogen Helicobacter pylori produce a type IV secretion system (cagT4SS) to inject the oncoprotein cytotoxin‐associated gene A (CagA) into gastric cells. This syringe‐like molecular apparatus is prolonged by an external pilus that exploits integrins as receptors to mediate the injection of CagA. The molecular determinants of the interaction of the cagT4SS pilus with the integrin ectodomain are still poorly understood. In this study, we have used surface plasmon resonance (SPR) to generate a comprehensive analysis of the protein–protein interactions between purified CagA, CagL, CagI, CagY repeat domain II (CagYRRII), CagY C‐terminal domain (CagYB10) and integrin α5β1 ectodomain (α5β1E) or headpiece domain (α5β1HP). We found that CagI, CagA, CagL and CagYB10 but not CagYRRII were able to interact with α5β1E with affinities similar to the one …
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T Koelblen, C Bergé, MV Cherrier, K Brillet… - The FEBS Journal, 2017