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Knowledge concerning in vitro regeneration and developmental responses of Carpobrotus edulis is limited. Adventitious shoots were regenerated from leaf explants on MS media supplemented with (0.05) μM TDZ. Aseptic seedlings were successfully raised on MS medium containing half strength salts. MS medium supplemented with 4.0 mg/L 2iP proved to be the best for multiple shoot induction. MS supplemented with 0.4 mg/L NAA was found suitable medium for root induction in excised micro-shoots. The plants were successfully acclimatized in the greenhouse at 100% survival rate. In vitro derived plants were analyzed using SDS-PAGE of soluble protein extracts and RAPD fingerprints. SDS-PAGE protein and randomly amplified polymorphic DNA (RAPD) analysis confirmed that all the regenerated plants from leaf and shoot tip were genetically identical to their donor plants, suggesting the absence of detectable genetic variation in the regenerated plants.[Salah Khattab and Fadia El Sherif. Effect of growth regulators on Carpobrotus edulis rapid micropropagation and molecular analysis.[Journal of American Science 2011; 7 (9): 511-520].(ISSN: 1545-1003). http://www. americanscience. org.