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Microbial fuel cell (MFC) is a budding technology for organic waste treatment with simultaneous bioelectricity generation. The present investigation explores the potential of pretreated sweet lime peel slurry as an anolyte with yeast and bacteria anode biocatalyst for bioenergy generation in MFCs. Sterilised cow urine was used as catholyte and Chlorella pyrenoidosa strain as cathode biocatalyst. Three H-shaped dual-chamber MFCs were fabricated using two plastic containers operating with no inoculum, Saccharomyces cerevisiae as only inoculum and co-culture of Saccharomyces cerevisiae with isolated cellulolytic bacteria, respectively, in the anode chamber. The anode was prepared using a rectangular stainless-steel mesh; the cathode was a cylindrical graphite rod. The maximum open-circuit voltages achieved by co-culture of bacteria and S. cerevisiae were 792.33 ± 1.53 mV and 481.33 ± 3.51 mV …