作者
Octavian Bucur, Bodvael Pennarun, Andreea Lucia Stancu, Monica Nadler, Maria Sinziana Muraru, Thierry Bertomeu, Roya Khosravi-Far
发表日期
2013/10
期刊
Apoptosis
卷号
18
页码范围
1154-1162
出版商
Springer US
简介
Successful translation of findings derived from preclinical studies into effective therapies is critical in biomedical research. Lack of robustness and reproducibility of the preclinical data, due to insufficient number of repeats, inadequate cell-based and mouse models contribute to the poor success rate. Antibodies are widely used in preclinical research, notably to determine the expression of potential therapeutic targets in tissues of interest, including tumors, but also to identify disease and/or treatment response biomarkers. We sought to determine whether the current antibody characterization standards in preclinical research are sufficient to ensure reliability of the data found in peer-reviewed publications. To address this issue, we used detection of the protein c-FLIP, a major factor of resistance to apoptosis, as a proof of concept. Accurate detection of endogenous c-FLIP levels in the preclinical settings is …
学术搜索中的文章
Poor antibody validation is a challenge in biomedical research: a case study for detection of c-FLIP
O Bucur, B Pennarun, AL Stancu, M Nadler, MS Muraru… - Apoptosis, 2013