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In present societies, knowledge is known as the main source of Economic prosperity and Societies that derive their economical power from the production and diffusion of information and knowledge are referred to as knowledge-based societies or economies. This paper aimed to measure Triple Helix for studying the innovation infrastructure in Iran in compare with Netherlands, Russia, and Turkey. This research is based on Webometrics methods and we performed this research in two ways: first, we used the number of hits and co-occurrence of “university”,“industry” and “government”. Second, we confined our search to Rich Files. In first way; the results show that in selected countries,“University”,“Industry” And “Government” are more integrated in Netherlands following by Russia, Turkey and Iran in recent years. Iran in compare with other countries has no a good situation. In second way; the results show a different situation. Netherlands has higher value in this indicator, following by Turkey, Iran and Russia.