作者
Andrew Olaf Shelton, Zachary J Gold, Alexander J Jensen, Erin D′ Agnese, Elizabeth Andruszkiewicz Allan, Amy Van Cise, Ramón Gallego, Ana Ramón‐Laca, Maya Garber‐Yonts, Kim Parsons, Ryan P Kelly
发表日期
2023/2
期刊
Ecology
卷号
104
期号
2
页码范围
e3906
出版商
John Wiley & Sons, Inc.
简介
Amplicon‐sequence data from environmental DNA (eDNA) and microbiome studies provide important information for ecology, conservation, management, and health. At present, amplicon‐sequencing studies—known also as metabarcoding studies, in which the primary data consist of targeted, amplified fragments of DNA sequenced from many taxa in a mixture—struggle to link genetic observations to the underlying biology in a quantitative way, but many applications require quantitative information about the taxa or systems under scrutiny. As metabarcoding studies proliferate in ecology, it becomes more important to develop ways to make them quantitative to ensure that their conclusions are adequately supported. Here we link previously disparate sets of techniques for making such data quantitative, showing that the underlying polymerase chain reaction mechanism explains the observed patterns of amplicon …
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AO Shelton, ZJ Gold, AJ Jensen, ED′ Agnese… - Ecology, 2023