查看文章

A simple technique for comparing and quantifying the ventilation capacity of vessels used for plant tissue culture is described. Ethylene was injected into culture vessels and its rate of loss monitored by gas chromatography. From the resulting exponential decay curves, the time in hours for half the ethylene to be lost (t₅₀) was calculated and used to compare different containers and sealing methods. Cultures of Ficus lyrata Warb. and Gerbera jamesonii Bolus grown for up to 28 d in plastic vessels sufficiently well-sealed to generate t₅₀ values of approx. 16 h, accumulated ethylene and carbon dioxide in association with depleted oxygen. The relationship between carbon dioxide accumulation and oxygen depletion within culture vessels indicated little if any anaerobic respiration. Gerbera explants did not appear to be affected by these gaseous environments. However, in Ficus, leaf expansion was approximately …