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As part of a continuing study concerning the analysis of phenolic compounds from quince fruit (Andrade et al., 1998; Silva et al., 2002 and references therein), we have been interested in screening the flavonoids in quince seed. From a preliminary examination of these compounds by reversed-phase HPLC, UV spectra and resistance to acidic hydrolysis, it was deduced that a number of C-glycosyl flavones were present. However, the analysis of this class of compound is not only difficult but was exacerbated in the present case due to the scarcity of plant material.

The use of MS in the analysis of flavonoids and their glycosides has been recently reviewed (Stobiecki, 2000). When this technique was first applied to the study of the non-volatile and thermally labile glycosyl flavonoids, a derivatisation procedure was necessary prior to analysis by electron impact (EI)/MS. In such cases permethylation (or per …