查看文章

Although it is well established that human adipose tissue (AT) shows circadian rhythmicity, published studies have been discussed as if tissues or systems showed only one or few circadian rhythms at a time. To provide an overall view of the internal temporal order of circadian rhythms in human AT including genes implicated in metabolic processes such as energy intake and expenditure, insulin resistance, adipocyte differentiation, dyslipidemia, and body fat distribution. Visceral and subcutaneous abdominal AT biopsies (n = 6) were obtained from morbid obese women (BMI ≥ 40 kg/m²). To investigate rhythmic expression pattern, AT explants were cultured during 24‐h and gene expression was analyzed at the following times: 08:00, 14:00, 20:00, 02:00 h using quantitative real‐time PCR. Clock genes, glucocorticoid metabolism‐related genes, leptin, adiponectin and their receptors were studied. Significant …