作者
Ruben F Pereira, Bianca N Lourenço, Paulo J Bártolo, Pedro L Granja
发表日期
2021/1
期刊
Advanced Healthcare Materials
卷号
10
期号
2
页码范围
2001176
简介
The properties of the surrounding cell environment are major determinants of cell response in 3D. However, the ability to unravel how these cues dictate the biological function in bioprinted constructs is limited by the lack of extracellular matrix (ECM)‐mimetic bioinks with fully controllable properties. In this study, a multifunctional bioink that uniquely combines the independent control over the biochemical and biophysical cues that regulate cell fate with the bioorthogonal nature of thiol–norbornene photoclick chemistry is designed for the extrusion bioprinting of bioinspired 3D cellular niches with tunable properties. The bioink rheology is controlled by ionic gelation, being dependent on both the type and content of divalent ions (calcium and barium), while the mechanical and biochemical properties of hydrogels are tailored via a post printing thiol–ene reaction. Bioprinted cell‐adhesive and protease‐degradable …
学术搜索中的文章
RF Pereira, BN Lourenço, PJ Bártolo, PL Granja - Advanced Healthcare Materials, 2021