作者
Krzysztof Bryniarski, Wlodzimierz Ptak, Asha Jayakumar, Kerstin Püllmann, Michael J Caplan, Arthit Chairoungdua, Jun Lu, Brian D Adams, Emilia Sikora, Katarzyna Nazimek, Susanna Marquez, Steven H Kleinstein, Panjamaporn Sangwung, Yasuko Iwakiri, Eric Delgato, Frank Redegeld, Bart R Blokhuis, Jacek Wojcikowski, Anna Wladyslawa Daniel, Tom Groot Kormelink, Philip W Askenase
发表日期
2013/7/1
期刊
Journal of Allergy and Clinical Immunology
卷号
132
期号
1
页码范围
170-181. e9
出版商
Mosby
简介
Background
T-cell tolerance of allergic cutaneous contact sensitivity (CS) induced in mice by high doses of reactive hapten is mediated by suppressor cells that release antigen-specific suppressive nanovesicles.
Objective
We sought to determine the mechanism or mechanisms of immune suppression mediated by the nanovesicles.
Methods
T-cell tolerance was induced by means of intravenous injection of hapten conjugated to self-antigens of syngeneic erythrocytes and subsequent contact immunization with the same hapten. Lymph node and spleen cells from tolerized or control donors were harvested and cultured to produce a supernatant containing suppressive nanovesicles that were isolated from the tolerized mice for testing in active and adoptive cell-transfer models of CS.
Results
Tolerance was shown due to exosome-like nanovesicles in the supernatants of CD8+ suppressor T cells that were not regulatory T …
学术搜索中的文章
K Bryniarski, W Ptak, A Jayakumar, K Püllmann… - Journal of Allergy and Clinical Immunology, 2013