作者
Benjamin dZ Silverman, William Fuller, Philip Eaton, Juelin Deng, J Randall Moorman, Joseph Y Cheung, Andrew F James, Michael J Shattock
发表日期
2005/1/1
期刊
Cardiovascular research
卷号
65
期号
1
页码范围
93-103
出版商
Elsevier Science
简介
Objective: The mechanism by which the cardiac Na/K ATPase (NKA) is regulated by phosphorylation is controversial. We have used the perforated-patch technique to limit cell dialysis and maintain conditions as near physiological as possible.
Methods: NKA pump current (Ip) was measured in isolated guinea pig ventricular myocytes, and its components (Iα1 and Iα2) defined by their differing dihydroouabain sensitivities.
Results: Treatment with 1 μmol/l forskolin for 4 min at 35 °C caused a significant increase in Iα1 of 36 ± 15% (P<0.05, n=6), but no change in Iα2. The presence of the PKA selective inhibitor H89 (50 μmol/l) throughout the protocol blocked the effect of the forskolin on Iα1. Treatment with H89 alone did not change Iα1 or Iα2. Isoelectric focusing gels of the NKA α1 subunit demonstrated six charge states, which were unaltered following treatment with forskolin. Western …
引用总数
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