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Global warming often affects the greenhouse gas (GHG) emissions, yet the effect of simulated warming on GHG emissions from wheat fields, the major source of nitrous oxide (N₂O) emissions, is still unclear. Therefore, using a free-air temperature increase system, we conducted a two-year field experiment to explore the effects of simulated warming on N₂O and methane (CH₄) emissions as well as wheat yields in a rice-wheat rotation system. Our results showed that compared with the ambient control, warming increased the cumulative seasonal emissions of N₂O by 34.21 % (P < 0.05), but did not affect the seasonal CH₄ emissions in the two years. Soil C/N ratio, pH, nirK gene and (nirK+nirS)/nosZ ratio were the main causes affecting N₂O emissions. Warming reduced the wheat yield by 19.42 % mainly due to the reduction of panicles and shorter vegetative growth (P < 0.05). Greenhouse gas intensity of warming …