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USING PRIMARY ANTIBODIES AGAINST THE LINEAR EPITOPES GPI12 PROTEIN INSTEAD OF COMPLETE PROTEIN IN LEISHMANIA MAJOR USING WESTERN BLOT TECHNIQUE

作者

NEJAD ALMANI POOYA GHASEMI, IRAJ SHARIFI, BAHRAM KAZEMI, ZAHRA BABAEI, MOJGAN BANDEHPOUR, SAMIRA SALARI, DEZAKI EBRAHIM SAEDI, FARIDEH TOHIDI

发表日期

2015/1/1

期号

简介

BACKGROUND AND AIM: IN WESTERN BLOT TING TECHNIQUE USUALLY THE PRIMARY POLYCLONAL ANTIBODIES AFTER INJECTION OF COMPLETE PROTEIN IN A SUITABLE HOST IS USED. HOWEVER, WE HAVE USED A NEW METHOD IN OUR OWN TEST. WESTERN BLOT ANALYSIS OF PROTEINS AFTER SDS PAGE LINEAR PROTEIN EPITOPES WAS EXPOSED TO THE PRIMARY ANTIBODY. WE HAVE ALSO TAKEN ADVANTAGE OF THE SAME POINT...

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USING PRIMARY ANTIBODIES AGAINST THE LINEAR EPITOPES GPI12 PROTEIN INSTEAD OF COMPLETE PROTEIN IN LEISHMANIA MAJOR USING WESTERN BLOT TECHNIQUE

NAP GHASEMI, I SHARIFI, B KAZEMI, Z BABAEI… - 2015