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A new HPLC method was developed for the estimation of carboxylic acid metabolite of clopidogrel bisulfate in rat plasma using atorvastatin as internal standard. Plasma samples were extracted with a mixture of ethyl acetate and di-chloro methane (80:20, v/v) followed by subsequent reconstitution in a mixture of water:methanol:acetonitrile (40:40:20, v/v). The chromatographic separation was achieved with gradient elution on Kromasil ODS, 250mm×4.6mm i.d., 5μm analytical column maintained at 30°C. Carboxylic acid metabolite of clopidogrel as well as the internal standard were detected at a wavelength of 220nm. The method was validated as per USFDA guidelines. Calibration curves were linear in the concentration range of 125.0–32,000ng/ml and the correlation coefficient was better than 0.999. The extraction efficiency for the carboxylic acid metabolite of clopidogrel was more than 85.76%. The intra-day …