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Olive (Olea europaea L.) breeding programs are hampered by the long juvenile period shown by this species. Genetic transformation would allow incorporation of interesting agronomical traits in much shorter times. Our group has developed an Agrobacterium-mediated transformation protocol for this species using embryogenic cultures as explants. In this protocol, somatic embryos derived from radicle explants are co-cultured with A. tumefaciens strain AGL1 containing the binary vector pBINUbiGUSint with the nptII gene for kanamycin selection and uidA gene as reporter. After 12 weeks of selection in the presence of 200 mg/L paromomycin, an average transformation rate of 17.5±5.2 was obtained, based on the number of infected explants proliferating in the selection medium. Almost 32% of the paramomycin resistant calli showed GUS activity. Somatic embryos from different transgenic lines were maturated and …