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The Trolox Equivalent Antioxidant Capacity (TEAC) assay is based on the scavenging of the 2,2′-azinobis-(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) radical (ABTS^•) converting it into a colorless product. The degree of decolorization induced by a compound is related to that induced by trolox, giving the TEAC value. The assay is frequently used for constructing structure activity relationships (SARs). HPLC analysis of the reaction mixture, obtained after scavenging of ABTS^• by the flavonoid chrysin, shows that a product is formed that also reacts with ABTS^•. The product has a higher antioxidant capacity and reacts faster with ABTS^• than the parent compound, chrysin. In contrast to the reaction product of chrysin, the reaction product of trolox, which is formed during scavenging of ABTS^•, i.e. trolox quinone, does not react with ABTS^•. The experiments show that the TEAC is the antioxidant capacity of the parent …