作者
Jesús Delgado‐Calle, Agustín F Fernández, Jesús Sainz, María T Zarrabeitia, Carolina Sañudo, Raúl García‐Renedo, María I Pérez‐Núñez, Carmen García‐Ibarbia, Mario F Fraga, José A Riancho
发表日期
2013/1
期刊
Arthritis & Rheumatism
卷号
65
期号
1
页码范围
197-205
出版商
Wiley Subscription Services, Inc., A Wiley Company
简介
Objective
To determine genome‐wide methylation profiles of bone from patients with hip osteoarthritis (OA) and those with osteoporotic (OP) hip fractures.
Methods
Trabecular bone pieces were obtained from the central part of the femoral head of 27 patients with hip fractures and 26 patients with hip OA. DNA was isolated, and methylation was explored with Illumina methylation arrays. RNA was extracted, pooled, and deep‐sequenced to obtain the whole transcriptome. Differentially methylated regions were identified, and connections between genes with differentially methylated regions were explored by pathway and text‐mining analyses.
Results
After quality control, methylation of 23,367 CpG sites (13,463 genes) was analyzed. There was a genome‐wide inverse relationship between methylation and gene expression in both patient groups. Comparison of OP and OA bones revealed 241 CpG sites, located …
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J Delgado‐Calle, AF Fernández, J Sainz… - Arthritis & Rheumatism, 2013
