作者
Hong Yu, Liam J Worrall, Thorsten Berger, Martin Petric, Bryan H Lin, Marija Vuckovic, Craig S Robb, Quan Le, Calem Kenward, Chuanbin Dai, Andrew Wakeham, Shaofeng Liu, Bryan Snow, Chantal Tobin, Patrick Budylowski, Furkan Guvenc, Annick You-Ten, Jillian Haight, Jennifer Silvester, Rashim Pal Singh, Sang Kyun Ahn, Azmiri Sultana, Betty Poon, Jessica Lam, Natasha Christie-Holmes, Mario Ostrowski, Scott D Gray-Owen, Shawn Kubli, Tak Mak, Natalie CJ Strynadka, Robert C Brunham
发表日期
2023/9/15
期刊
The Journal of Immunology
简介
Current vaccine efforts to combat SARS-CoV-2 are focused on the whole spike protein administered as mRNA, viral vector, or protein subunit. However, the SARS-CoV-2 receptor-binding domain (RBD) is the immunodominant portion of the spike protein, accounting for 90% of serum neutralizing activity. In this study, we constructed several versions of RBD and together with aluminum hydroxide or DDA (dimethyldioctadecylammonium bromide)/TDB (D-(+)-trehalose 6, 69-dibehenate) adjuvant evaluated immunogenicity in mice. We generated human angiotensin-converting enzyme 2 knock-in mice to evaluate vaccine efficacy in vivo following viral challenge. We found that 1) subdomain (SD) 1 was essential for the RBD to elicit maximal immunogenicity; 2) RBDSD1 produced in mammalian HEK cells elicited better immunogenicity than did protein produced in insect or yeast cells; 3) RBDSD1 combined with the CD4 …
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H Yu, LJ Worrall, T Berger, M Petric, BH Lin… - The Journal of Immunology, 2023