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Due to the non-stationary, multicomponent nature of biomedical signals, the use of time-frequency analysis can be inevitable for these signals. The choice of the proper time-frequency distribution (TFD) that can reveal the exact multicomponent structure of biological signals is vital in many applications, including the diagnosis of medical abnormalities. In this paper, the instantaneous frequency (IF) estimation using four well-known TFDs is applied for analyzing biological signals. These TFDs are: the Wigner–Ville distribution (WVD), the Choi–Williams distribution (CWD), the Exponential T-distribution (ETD) and the Hyperbolic T-distribution (HTD). Their performance over normal and abnormal biological signals as well as over multicomponent frequency modulation (FM) signals in additive Gaussian noise was compared. Moreover, the feasibility of utilizing the wavelet transform (WT) in IF estimation is also studied. The …