作者
Jean-Christophe Lagier, Saber Khelaifia, Maryam Tidjani Alou, Sokhna Ndongo, Niokhor Dione, Perrine Hugon, Aurelia Caputo, Frédéric Cadoret, Sory Ibrahima Traore, El Hadji Seck, Gregory Dubourg, Guillaume Durand, Gaël Mourembou, Elodie Guilhot, Amadou Togo, Sara Bellali, Dipankar Bachar, Nadim Cassir, Fadi Bittar, Jérémy Delerce, Morgane Mailhe, Davide Ricaboni, Melhem Bilen, Nicole Prisca Makaya Dangui Nieko, Ndeye Mery Dia Badiane, Camille Valles, Donia Mouelhi, Khoudia Diop, Matthieu Million, Didier Musso, Jônatas Abrahão, Esam Ibraheem Azhar, Fehmida Bibi, Muhammad Yasir, Aldiouma Diallo, Cheikh Sokhna, Felix Djossou, Véronique Vitton, Catherine Robert, Jean Marc Rolain, Bernard La Scola, Pierre-Edouard Fournier, Anthony Levasseur, Didier Raoult
发表日期
2016/12
期刊
Nature microbiology
卷号
1
期号
12
出版商
Springer Science and Business Media LLC
简介
Metagenomics revolutionized the understanding of the relations among the human microbiome, health and diseases, but generated a countless number of sequences that have not been assigned to a known microorganism 1. The pure culture of prokaryotes, neglected in recent decades, remains essential to elucidating the role of these organisms 2. We recently introduced microbial culturomics, a culturing approach that uses multiple culture conditions and matrix-assisted laser desorption/ionization–time of flight and 16S rRNA for identification 2. Here, we have selected the best culture conditions to increase the number of studied samples and have applied new protocols (fresh-sample inoculation; detection of microcolonies and specific cultures of Proteobacteria and microaerophilic and halophilic prokaryotes) to address the weaknesses of the previous studies 3–5. We identified 1,057 prokaryotic species, thereby …
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JC Lagier, S Khelaifia, MT Alou, S Ndongo, N Dione… - Nature microbiology, 2016