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Using a reverse transcription/polymerase chain reaction method, the temporal pattern of expression of the myogenic regulatory genes (myf5, myogenin, MRF4, myo D) was quantitated in developing mouse muscle (whole embryo: 6.5 to 12.5 days postcoitum (dpc); front limb buds: 9.5 to 12.5 dpc; hind limb buds: 11.5 to 14.5 dpc) and related to expression of TGF-β1, b-FGF, IGF-I, and IGF-II. Myf5 was the first myogenic regulatory factor to appear in both the whole embryo and front limb bud, with expression evident 7.5 and 9.5 dpc, respectively. A transient peak of MRF4 expression occurred 10.5 dpc in both the whole embryo and the front limb bud. Myogenin and myo D expression in the whole embryo was detected 8.5 and 9.5 dpc, respectively. In the front limb bud myogenin and myo D expression was not detected until 10.5 dpc. In the hind limb bud myf5, myogenin, and MRF4 expression was detected 11.5 dpc. Myo …