作者
Madson Q Almeida, Ibere Cauduro Soares, Tamaya C Ribeiro, Maria Candida BV Fragoso, Lidiane V Marins, Alda Wakamatsu, Rodrigo A Ressio, Mirian Y Nishi, Alexander AL Jorge, Antonio M Lerario, Venancio AF Alves, Berenice B Mendonca, Ana Claudia Latronico
发表日期
2010/3/1
期刊
The Journal of Clinical Endocrinology & Metabolism
卷号
95
期号
3
页码范围
1458-1462
出版商
Oxford University Press
简介
Background: Steroidogenic factor 1 (SF-1) is a key determinant of endocrine development and function of adrenal cortex. SF-1 overexpression and gene amplification were previously demonstrated in a small group of pediatric adrenocortical tumors.
Objective: Our objective was to determine the frequency of SF-1 protein expression and gene amplification in a large cohort of pediatric and adult adrenocortical tumors.
Patients: SF-1 protein expression was assessed in a cohort of 103 adrenocortical tumors from 36 children and 67 adults, whereas gene amplification was studied in 38 adrenocortical tumors (17 from children).
Methods: Tissue microarray, multiplex ligation-dependent probe amplification, and quantitative real-time PCR were used.
Results: A strong nuclear SF-1 expression was detected by tissue microarray in 56% (20 of 36) and 19% (13 of 67) of the …
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MQ Almeida, IC Soares, TC Ribeiro, MCBV Fragoso… - The Journal of Clinical Endocrinology & Metabolism, 2010