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The prevalence of bovine mastitis was evaluated in 4.260 udder quarts from 1.065 cows located in 15 cattle farms managed as dual purpose systems (beef and dairy) in Monteria (Cordoba, Colombia). The farms were chosen using a non-random probability sampling design. To isolate the microorganisms involved in mastitis, milk samples were taken from the quarts with trace reaction, as well as from sub-clinical or clinically affected quarts. A high percentage (92.21%) of isolated samples included infectious organisms. The main pathogen isolated was Staphylococcus aureus (87.56% of samples), while Streptococuss uberis, Corynebacterium bovis, Streptococcus agalactiae, and coagulase-negative Staphylococcus (SCN) were found in 3.60, 2.10, 2.10, and 0.3% of samples, respectively. We conclude that Staphylococcus aureus, the main etiological agent, is sensitive to antibiotics commonly used in the prevention and control of mastitis on dual purpose systems in this region.