作者
Ricardo J Rodrigues, Teresa Almeida, Peter J Richardson, Catarina R Oliveira, Rodrigo A Cunha
发表日期
2005/7/6
期刊
Journal of Neuroscience
卷号
25
期号
27
页码范围
6286-6295
出版商
Society for Neuroscience
简介
ATP is released in a vesicular manner from nerve terminals mainly at higher stimulation frequencies. There is a robust expression of ATP (P2) receptors in the brain, but their role is primarily unknown. We report that ATP analogs biphasically modulate the evoked release of glutamate from purified nerve terminals of the rat hippocampus, the facilitation being mediated by P2X1, P2X2/3, and P2X3 [antagonized by 8-(benzamido)naphthalene-1,3,5-trisulfonate and 2′,3′-O-(2,4,6-trinitrophenyl)-ATP] and the inhibition by P2Y1, P2Y2, and/or P2Y4 [antagonized by reactive blue 2 and 2′deoxy-N6-methyladenosine-3′,5′-bisphosphate and mimicked by P1-(urinine 5′-),P4-(inosine 5′-) tetraphosphate and 2-methylthio-ADP] receptors. The combination of single-cell PCR analysis of rat hippocampal pyramidal neurons, Western blot analysis of purified presynaptic active zone fraction, and immunocytochemical …
引用总数
学术搜索中的文章
RJ Rodrigues, T Almeida, PJ Richardson, CR Oliveira… - Journal of Neuroscience, 2005