作者
Rodrigo A Cunha, E Sylvester Vizi, J Alexandre Ribeiro, Ana M Sebastião
发表日期
1996/11
期刊
Journal of neurochemistry
卷号
67
期号
5
页码范围
2180-2187
出版商
Blackwell Science Ltd
简介
The release of adenosine and ATP evoked by electrical field stimulation in rat hippocampal slices was investigated with the following two patterns of stimulation: (1) a brief, high‐frequency burst stimulation (trains of stimuli at 100 Hz for 50 ms applied every 2 s for 1 min), to mimic a long‐term potentiation (LTP) stimulation paradigm, and (2) a more prolonged (3 min) and low‐frequency (5 Hz) train stimulation, to mimic a long‐term depression (LTD) stimulation paradigm. The release of ATP was greater at a brief, high‐frequency burst stimulation, whereas the release of [3H]adenosine was slightly greater at a more prolonged and low‐frequency stimulation. To investigate the source of extracellular adenosine, the following two pharmacological tools were used; α,β‐methylene ADP (AOPCP), an inhibitor of ecto‐5′‐nucleotidase, to assess the contribution of the catabolism of released adenine nucleotides as a source of …
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RA Cunha, ES Vizi, JA Ribeiro, AM Sebastião - Journal of neurochemistry, 1996