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• Premise of the study: We explored a targeted enrichment strategy to facilitate rapid and lowcost nextgeneration sequencing (NGS) of numerous complete plastid genomes from across the phylogenetic breadth of angiosperms.

• Methods and Results: A custom RNA probe set including the complete sequences of 22 previously sequenced eudicot plastomes was designed to facilitate hybridizationbased targeted enrichment of eudicot plastid genomes. Using this probe set and an Agilent SureSelect targeted enrichment kit, we conducted an enrichment experiment including 24 angiosperms (22 eudicots, two monocots), which were subsequently sequenced on a single lane of the Illumina GAIIx with singleend, 100bp reads. This approach yielded nearly complete to complete plastid genomes with exceptionally high coverage (mean coverage: 717×), even for the two monocots.

• Conclusions: Our enrichment …