查看文章

Second-generation bioethanol production by a newly isolated thermotolerant yeast strain was studied at 42 °C and above using pilot-scale dilute acid pretreated wheat straw (WS) as feedstock. This strain was identified as Kluyveromyces marxianus DBTIOC-35 by biochemical characterization as well as molecular phylogenetic analysis of the ITS-5.8S rRNA gene and D1/D2 domain of the 26S rRNA gene after PCR amplification and sequencing. Simultaneous saccharification and fermentation (SSF) at 42 °C and 45 °C using 10% biomass loading resulted in ethanol titers of 29.0 and 16.1 g L−1, respectively. At 42 °C ethanol productivity was higher during SSF (0.92 g L−1 h−1) than separate hydrolysis and fermentation (SHF) (0.49 g L−1 h−1) at 20% biomass loading. The results indicated that at 20% biomass loading, SSF without pre-saccharification led to more ethanol production (66.2 g L−1 with 83.3% yield) at a …