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Endangered woundfin Plagopterus argentissimus embryos and larvae were exposed to artificial ultraviolet-B (UV-B) radiation to directly examine the effects on mortality. The experiment was part of a project assisting the Virgin River Resource Management and Recovery Program's efforts to increase hatchery production of this endangered fish. The UV-B radiation used in this experiment was administered in treatments of 0.060, 0.030, and 0.015 mW/cm² to simulate 100, 50, and 25% of the ambient irradiance levels documented in outdoor tanks and living streams at Bubbling Ponds State Fish Hatchery, in Arizona. Embryos and larvae were exposed for 14.5 h followed by 9.5 h of darkness, in correspondence with the daylight hours at Bubbling Ponds. No embryos survived UV-B treatments; mortality among control (UV-B–free) treatments varied (5–100%) among females, indicating that there may be important …