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Because of the difficulty in resolving the large variability of N₂ fixation with current methods which rely on discrete sampling, the development of new methods for high-resolution measurements is highly desirable. We present a new method for high-frequency measurements of aquatic N₂ fixation by continuous flow-through incubations and spectral monitoring of the acetylene (C₂H_2, a substrate analog for N₂) reduction to ethylene (C₂H₄). In this method, named Flow-through Incubation Acetylene Reduction Assays by Cavity Ring-Down Laser Absorption Spectroscopy (FARACAS), dissolved C₂H₂ is continuously admixed with seawater upstream of a continuous-flow stirred-tank reactor (CFSR) in which C₂H₂ reduction takes place. Downstream of the flow-through incubator, the C₂H₄ gas is stripped using a bubble column contactor and circulated with a diaphragm pump into a wavelength-scanned cavity ring down …