作者
Mathurin Flamant, Philippe Aubert, Malvyne Rolli-Derkinderen, Arnaud Bourreille, Margarida Ribeiro Neunlist, Maxime M Mahé, Guillaume Meurette, Benoit Marteyn, Tor Savidge, Jean Paul Galmiche, Philippe J Sansonetti, Michel Neunlist
发表日期
2011/4/1
期刊
Gut
卷号
60
期号
4
页码范围
473-484
出版商
BMJ Publishing Group
简介
Background
Enteric glial cells (EGCs) are important regulators of intestinal epithelial barrier (IEB) functions. EGC-derived S-nitrosoglutathione (GSNO) has been shown to regulate IEB permeability. Whether EGCs and GSNO protect the IEB during infectious insult by pathogens such as Shigella flexneri is not known.
Methods
S flexneri effects were characterised using in vitro coculture models of Caco-2 cells and EGCs (or GSNO), ex vivo human colonic mucosa, and in vivo ligated rabbit intestinal loops. The effect of EGCs on S flexneri-induced changes in the invasion area and the inflammatory response were analysed by combining immunohistochemical, ELISA and PCR methods. Expression of small G-proteins was analysed by western blot. Expression of ZO-1 and localisation of bacteria were analysed by fluorescence microscopy.
Results
EGCs significantly reduced barrier lesions and inflammatory response …
学术搜索中的文章
M Flamant, P Aubert, M Rolli-Derkinderen, A Bourreille… - Gut, 2011