作者
Sonika Saddar, Véronique Carriere, Wan-Ru Lee, Keiji Tanigaki, Ivan S Yuhanna, Sajesh Parathath, Etienne Morel, Manya Warrier, Janet K Sawyer, Robert D Gerard, Ryan E Temel, J Mark Brown, Margery Connelly, Chieko Mineo, Philip W Shaul
发表日期
2013/1/4
期刊
Circulation research
卷号
112
期号
1
页码范围
140-151
出版商
Lippincott Williams & Wilkins
简介
Rationale:
Signal initiation by the high-density lipoprotein (HDL) receptor scavenger receptor class B, type I (SR-BI), which is important to actions of HDL on endothelium and other processes, requires cholesterol efflux and the C-terminal transmembrane domain. The C-terminal transmembrane domain uniquely interacts with plasma membrane (PM) cholesterol.
Objective:
The molecular basis and functional significance of SR-BI interaction with PM cholesterol are unknown. We tested the hypotheses that the interaction is required for SR-BI signaling, and that it enables SR-BI to serve as a PM cholesterol sensor.
Methods and Results:
In studies performed in COS-M6 cells, mutation of a highly conserved C-terminal transmembrane domain glutamine to alanine (SR-BI-Q445A) decreased PM cholesterol interaction with the receptor by 71% without altering HDL binding or cholesterol uptake or efflux, and it yielded a …
学术搜索中的文章
S Saddar, V Carriere, WR Lee, K Tanigaki, IS Yuhanna… - Circulation research, 2013