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Current trends and future innovative issues focus on the development of smart multifunctional bio-nanostructures. In this study, novel maca extract-coated CuO nanoparticles (MaE-CuO NPs) were prepared using a green and facile sonication method. Multifunctional MaE-CuO NPs were characterized by different techniques, such as Scanning Electron Microscopy (SEM), Energy Dispersive X-ray Spectroscopy (EDX), High Resolution Transmission Electron Microscopy (HRTEM), Fourier Transform Infrared (FTIR) spectroscopy, X-ray diffraction (XRD), and Brunauer–Emmett–Teller (BET) techniques. According to the results, we proved that the sonosynthesized MaE-CuO NPs had a uniform spherical shape with an average diameter range of ∼ 10 nm, while the surface area was 344.645 m²/g. The preservative performance of the antimicrobial MaE-CuO NPs was investigated to evaluate the biological activity of the …