作者
Diana Hide, Martí Ortega-Ribera, Juan-Carlos Garcia-Pagan, Carmen Peralta, Jaime Bosch, Jordi Gracia-Sancho
发表日期
2016/2/24
期刊
Scientific Reports
卷号
6
期号
1
页码范围
22107
出版商
Nature Publishing Group UK
简介
Warm ischemia and reperfusion (WIR) causes hepatic damage and may lead to liver failure, however the mechanisms involved are largely unknown. Here we have characterized the microcirculatory status and endothelial phenotype of livers undergoing WIR and evaluated the use of simvastatin in WIR injury prevention. Male Wistar rats received simvastatin, or vehicle, 30 min before undergoing 60 min of partial warm ischemia (70%) followed by 2 h or 24 h of reperfusion. Hepatic and systemic hemodynamics, liver injury (AST, ALT, LDH), endothelial function (vasodilatation in response to acetylcholine), KLF2 and nitric oxide pathways, oxidative stress, inflammation (neutrophil and macrophage infiltration) and cell death were evaluated. Profound microcirculatory dysfunction occurred rapidly following WIR. This was evidenced by down-regulation of the KLF2 vasoprotective pathway, impaired vasodilatory …
学术搜索中的文章
D Hide, M Ortega-Ribera, JC Garcia-Pagan, C Peralta… - Scientific Reports, 2016